double polymerase chain reaction (nested-pcr) Search Results


nested reverse transcription polymerase chain reaction (rt-pcr) assay  (DaAn Gene)
90
DaAn Gene nested reverse transcription polymerase chain reaction (rt-pcr) assay
Nested Reverse Transcription Polymerase Chain Reaction (Rt Pcr) Assay, supplied by DaAn Gene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
nested reverse transcription polymerase chain reaction (rt-pcr) assay - by Bioz Stars, 2026-08
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nested polymerase chain reaction (pcr)  (Alpha Biolaboratory)
90
Alpha Biolaboratory nested polymerase chain reaction (pcr)
Nested Polymerase Chain Reaction (Pcr), supplied by Alpha Biolaboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nested polymerase chain reaction (pcr) - by Bioz Stars, 2026-08
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nested polymerase chain reaction (pcr  (Becton Dickinson)
90
Becton Dickinson nested polymerase chain reaction (pcr
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Polymerase Chain Reaction (Pcr, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/pmc00266778-37-21-13?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
nested polymerase chain reaction (pcr - by Bioz Stars, 2026-08
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nested polymerase chain reaction (pcr)  (Wyeth Biopharma)
90
Wyeth Biopharma nested polymerase chain reaction (pcr)
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Polymerase Chain Reaction (Pcr), supplied by Wyeth Biopharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/10__1097_slash_jcp__0b013e318192e4c1-71-53-80?v=Wyeth+Biopharma
Average 90 stars, based on 1 article reviews
nested polymerase chain reaction (pcr) - by Bioz Stars, 2026-08
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hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr)  (CollaGenex Pharmaceuticals)
90
CollaGenex Pharmaceuticals hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr)
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Hiv Detection In Subgingival And Salivary Specimens By Nested Reverse Transcription Polymerase Chain Reaction (Nrt Pcr), supplied by CollaGenex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/10__1902_slash_jop__1994__65__10__976-377-13-8?v=CollaGenex+Pharmaceuticals
Average 90 stars, based on 1 article reviews
hiv detection in subgingival and salivary specimens by nested reverse transcription polymerase chain reaction (nrt-pcr) - by Bioz Stars, 2026-08
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nested real-time polymerase chain reaction (rt-pcr) of pml/rara  (Informa UK Limited)
90
Informa UK Limited nested real-time polymerase chain reaction (rt-pcr) of pml/rara
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Real Time Polymerase Chain Reaction (Rt Pcr) Of Pml/Rara, supplied by Informa UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/pm27658340-27-12-31?v=Informa+UK+Limited
Average 90 stars, based on 1 article reviews
nested real-time polymerase chain reaction (rt-pcr) of pml/rara - by Bioz Stars, 2026-08
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nested polymerase chain reaction-sequence specific primer (pcr-ssp) method  (Informa UK Limited)
90
Informa UK Limited nested polymerase chain reaction-sequence specific primer (pcr-ssp) method
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Nested Polymerase Chain Reaction Sequence Specific Primer (Pcr Ssp) Method, supplied by Informa UK Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/pm27923274-22-2-30?v=Informa+UK+Limited
Average 90 stars, based on 1 article reviews
nested polymerase chain reaction-sequence specific primer (pcr-ssp) method - by Bioz Stars, 2026-08
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reverse transcription-nested polymerase chain reaction (rt-nested pcr)  (Neodin Medical Institute)
90
Neodin Medical Institute reverse transcription-nested polymerase chain reaction (rt-nested pcr)
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Reverse Transcription Nested Polymerase Chain Reaction (Rt Nested Pcr), supplied by Neodin Medical Institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/pm20064673-11-162-114?v=Neodin+Medical+Institute
Average 90 stars, based on 1 article reviews
reverse transcription-nested polymerase chain reaction (rt-nested pcr) - by Bioz Stars, 2026-08
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semi-nested polymerase chain-reaction (pcr) method with consensus vh (fr1c) and jh (ljh, vljh) primers  (Bioservice Scientific Laboratories)
90
Bioservice Scientific Laboratories semi-nested polymerase chain-reaction (pcr) method with consensus vh (fr1c) and jh (ljh, vljh) primers
Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by <t>PCR</t> with total rat testis RNA and a rat pachytene <t>spermatocyte</t> <t>cDNA</t> library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.
Semi Nested Polymerase Chain Reaction (Pcr) Method With Consensus Vh (Fr1c) And Jh (Ljh, Vljh) Primers, supplied by Bioservice Scientific Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/double+polymerase+chain+reaction+%28nested-pcr%29/pm12189505-37-17-24?v=Bioservice+Scientific+Laboratories
Average 90 stars, based on 1 article reviews
semi-nested polymerase chain-reaction (pcr) method with consensus vh (fr1c) and jh (ljh, vljh) primers - by Bioz Stars, 2026-08
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Image Search Results


Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by PCR with total rat testis RNA and a rat pachytene spermatocyte cDNA library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.

Journal:

Article Title: Acroplaxome, an F-Actin-Keratin-containing Plate, Anchors the Acrosome to the Nucleus during Shaping of the Spermatid Head

doi: 10.1091/mbc.E03-04-0226

Figure Lengend Snippet: Expression and localization of K5 in rat testis and skin using affinity purified antibodies. (A) Gene expression of K5 in rat testis and pachytene spermatocytes demonstrated by PCR with total rat testis RNA and a rat pachytene spermatocyte cDNA library. (B) A fusion protein of the cloned rat testis K5 cDNA (see MATERIALS AND METHODS) is recognized by anti-K5 sera. Two independent clones (1 and 2) under IPTG-inducing and noninducing conditions are shown. (C) Recognition of His-purified fusion protein by affinity-purified anti-K5 serum is blocked by fusion K5 peptide (lane 2). In contrast, an unrelated peptide does not affect antigen recognition (lane 3). Lanes 1 and 4, affinity-purified and not purified anti-K5 sera; lane 5, control (secondary alkaline phosphatase-conjugated anti-rabbit serum). Numbers at the left indicate the position of molecular mass (Mr) markers. (D–F) Immunohistochemical localization of K5 in rat (D, immunofluorescence) and human epidermis (F, immunoperoxidase) by using anti-rat K5 serum (D) and anti-mouse K5 serum (F). (E) Negative control (preimmune serum). The dotted line indicates the epidermis-dermis boundary. The arrowhead denotes the outer layer of the epidermis. Bars (D–F), 100 μm. (G) Immunoblot showing the equivalent molecular mass of rat testis and human skin (epidermis) K5.

Article Snippet: We started cloning of Sak57 cDNA from a rat testis λgt11 cDNA library (BD Biosciences Clontech, Palo Alto, CA) by using nested polymerase chain reaction (PCR) with primers gt11-5′ and R followed by gt11-5′ (GACTCCTGGAGCCCG) and T. Sequencing of an obtained fragment revealed near identity to the mRNA for human keratin 5 (K5).

Techniques: Expressing, Affinity Purification, cDNA Library Assay, Clone Assay, Purification, Immunohistochemical staining, Immunofluorescence, Negative Control, Western Blot